Genetic analysis of yield components under drought conditions and QTL mapping for drought tolerance in soybean [Glycine max (L.) Merr.]

Inquiry Proposal

  1. Title of contemplated inquiry project:

Genetic segregation of relinquish components subordinate thirst provisions and QTL mapping coercion thirst tolerance in soybean [Glycine max (L.) Merr.]


  1. Summary of inquiry

An estimated 40% of soybean [Glycine max (L.) Merr.] coercionmation worldwide is obsolete each year due to thirst and such losses may aid acception as thirsts befit over many and rigid consequently of clime modify. That is why the fruit of soybean thirst-indulgent varieties befits increasingly over grave. We earn act a phenotypic characterization of soybean population extraneous from a morose betwixt thirst-indulgent genotype and thirst-perceptive genotype subordinate twain well-watered (WW) and insinuate-stressed (WS) provisions. Articulation linkage vital stroke loci (QTL) mapping earn be acted to expose the genomic regions that administer soybean thirst tolerance subordinate irreferring-to insinuate regimes. Finally, Genome-Wide Conjunction Studies (GWAS) earn be acted to authenticate the applicant genes associated with relinquish components subordinate thirst provisions. This inquiry gift to introduce thirst tolerance QTLs and applicant genes coercion soybean philosophical brotherhood and let-go a symbolical oblation to the manners endeavor coercion soybean thirst tolerance.

Keywords: Soybean [Glycine max (L.) Merr.], Relinquish components, Thirst tolerance, Vital stroke loci

  1. Introduction

Thirst is individual of the dignified constraints to soybean [Glycine max (L.) Merr.] coercionmation in China and worldwide. Up to 40% of soybean productivity is obsolete consequently of thirst [1] and such losses may aid acception as thirsts befit over many and rigid consequently of clime modify [2]. Irrigation is referable an liberty coercion wide quantity of farmers and there is scant virtual coercion any expatiation of irrigation in developing countries [3].

The conservation of genetics to reform thirst tolerance and let-go relinquish arrestation is an grave dissect of the breach to stabilizing global coercionmation. That is why the fruit of soybean varieties with enhanced tolerance to thirst importance and loftier insinuate conservation earningness (WUE) has befit a excellent pre-eminence motive coercion dignified manners programs, twain in the individual and exoteric sectors. The manners programs reform thirst tolerance via diverse strategies such as intermittent preference and evaluation of segregating population subordinate managed and multi-location thirst-importance environment, conservation of unimportant strokes coercion preference subordinate thirst mode, genomic-based adit and transgenic technology.

Great manners endeavors own made coercion thirst tolerance in soybean using irreferring-to relinquish components, unimportant strokes and molecular markers coercion preference. Mian et al. verified foul-mouthed QTL associated with WUE using 120 F4-extraneous soybean population from a morose Young × PI416937 [4]. In another examine, they patent clear a population of 116 F2-extraneous direction from a morose of S100 × Tokyo and reputed an attached QTL coercion WUE in soybean [5]. Over of-late, 40 QTLs including 17 coercion leaf insinuate standing strokes subordinate thirst importance and 23 coercion origin relinquish subordinate well-watered and thirst-stressed provisions were verified using soybean recombinant ingrained population with 184 F2:7:11 directions patent clear from a morose betwixt Kefeng1 (thirst indulgent) and Nannong1138-2 (thirst perceptive) [6]. Using the corresponding population, Du et al. own so reputed 19 QTLs associated with origin relinquish per insert and 10 QTLs associated with thirst irritability abjuration [7]. In a irreferring-to examine, Zhang et al. unnatural a designated of BC2F3 directions with Hongfeng 11 as intermittent committer and Harosoy as donor committer and expoorigin 18 QTLs of thirst tolerance at germination range in soybean [8]. Of-late, Khan et al. conservationd detested brace-range multi-locus genome-wide conjunction studies with a nested conjunction mapping population with 403 directions comprising brace recombinant ingrained direction populations: M8206 × TongShan and ZhengYang × M8206 to authenticate 73 and 38 QTLs  coercion the thirst tolerance strokes referring-to source protraction and referring-to twig protraction, respectively [9].

Although distinct studies own reputed QTLs coercion thirst tolerance in soybean, the QTL mapping results want to be validated antecedently application to cultivar fruit. That is why a odd examine is inevitable in ordain to let-go over counsel and symbolically co-opeblame to the sentence whole QTLs.

  1. Objectives

This examine gift to:

  • Act a phenotypic characterization of soybean population extraneous from a morose betwixt thirst-indulgent genotype and thirst sensitive genotype subordinate twain well-watered (WW) and insinuate-stressed (WS) provisions;
  • Act a Articulation linkage QTL mapping to expose the genomic regions that administer soybean thirst tolerance subordinate irreferring-to insinuate regimes;
  • Act a Genome-Wide Conjunction Studies (GWAS) to authenticate the applicant genes associated relinquish components subordinate thirst provisions.
  1. Material and Methods

Insert materials and opportunity trials

An F2 population extraneous from a morose betwixt thirst-indulgent genotype and thirst-sensitive genotype earn be conservationd to compose the genetic linkage map. The brace committers and the F2 inserts earn be evaluated twain subordinate opportunity and greenhoconservation provisions with brace insinuate regimes: well-watered (control) and thirst importance. The test earn be crystallized in a chance entire stop plan with three replicates.

Referring-to insinuate full and excised leaf insinuate losing blame and leaf wilting earn be measured in ordain to discover quenched the insinuate standing in inserts subordinate thirst importance. Origin relinquish per insert earn be measured in twain thirst-stressed and well-watered administer plots. The subjoined relinquish components earn be considered: compute of nodes per insert, compute of pods per node, compute of origins per pod and origin greatness.

Facts segregation

Using SAS software (SAS Institute, lnc.), we earn act a picturesque statistics segregation on the phenotypic facts in ordain to collate the strokes of the committers with the F2 inserts.

We earn outgrowth leaf samples from the committer genotypes and each F2 insert coercion DNA lineage using CTAB progress. Coercion Single Nucleotide Polymorphism (SNPs) genotyping, the restriction-site-conjunction DNA sequencing (RAD-seq) earn be conservationd. The genotypic facts earn be promotive into PowerMarker V3.25 [10] coercion vigilance of picturesque statistics. We earn evaluate the population composition using the software STRUCTURE 2.2 [11].

Pearson’s interrelation coefficients earn be congenial to designate relationships betwixt the sundry strokes subordinate examine. QTL segregation earn be acted using composite intermission mapping in WinQTL cartographer, statement 2.5 (

  1. Anticipated quenchedcome and prize of the inquiry

This examine earn expose QTLs subordinate well-watered and insinuate-stressed provisions by articulation linkage mapping in soybean F2 population. The genome-wide conjunction segregation earn authenticate SNPs associated with relinquish components subordinate thirst mode and dispose the SNPs in applicant genes. This inquiry gift to introduce thirst tolerance QTLs and applicant genes coercion soybean philosophical brotherhood and let-go a symbolical oblation to the manners endeavor coercion soybean thirst tolerance.

7. Literature cited

[1] D. T. Le et al., “Differential Gene Expression in Soybean Leaf Tissues at Late Fruital Ranges subordinate Thirst Importance Revealed by Genome-Wide Transcriptome Segregation,” PLoS Individual, vol. 7, no. 11, p. e49522, Nov. 2012.

[2] FAOSTAT, “Statistical Factsbase of the Food and Agriculture of the United Nations,” Rome, 2010.

[3] World Bank, “Agriculture Investment Sourcebook. Agriculture and Rural Fruit,” Washington DC, 2005.

[4] M. A. R. Mian et al., “Molecular Markers Associated with Insinuate Conservation Earningness and Leaf Ash in Soybean,” Crop Sci., vol. 36, no. 5, p. 1252, 1996.

[5] M. A. R. Mian, D. A. Ashley, and H. R. Boerma, “An Attached QTL coercion Insinuate Conservation Earningness in Soybean,” Crop Sci., vol. 38, no. 2, p. 390, 1998.

[6] W. Du, D. Yu, and S. Fu, “Detection of Vital Stroke Loci coercion Relinquish and Thirst Tolerance Strokes in Soybean Using a Recombinant Ingrained Direction Population,” J. Integr. Insert Biol., vol. 51, no. 9, pp. 868–878, Sep. 2009.

[7] W. Du, M. Wang, S. Fu, and D. Yu, “Mapping QTLs coercion origin relinquish and thirst irritability abjuration in soybean (Glycine max L.) amorose irreferring-to environments,” J. Genet. Genomics, vol. 36, no. 12, pp. 721–731, Dec. 2009.

[8] W. B. Zhang et al., “Dissection of genetic overlap of thirst and low-temperature tolerance QTLs at the germination range using backmorose introgression directions in soybean,” Mol. Biol. Rep., vol. 39, no. 5, pp. 6087–6094, May 2012.

[9] M. A. Khan, F. Tong, W. Wang, J. He, T. Zhao, and J. Gai, “Segregation of QTL–allele order conferring thirst tolerance at originling range in a nested conjunction mapping population of soybean [Glycine max (L.) Merr.] using a fantastic GWAS progress,” Planta, vol. 248, no. 4, pp. 947–962, Oct. 2018.

[10] K. Liu and S. V. Muse, “PowerMarker: an integrated segregation environment coercion genetic marker segregation,” Bioinformatics, vol. 21, no. 9, pp. 2128–2129, May 2005.

[11] J. K. Pritchard, M. Stephens, and P. Donnelly, “Inference of Population Composition Using Multilocus Genotype Facts,” Genetics, vol. 155, no. 2, pp. 945–959, 2000.

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